To your chromosome 4, the around three QTLs were during the 66
Package plots proving the results of one’s marker genotypic kinds (AAAA, AAAB, AABB, ABBB, BBBB) away from cuatro applicant SNPs into the tuber glucose attention (GLU); bud-end fry colour (FBE); stem-tuber fry color (FSE); ID, Idaho
The fresh co-localization from numerous QTLs within maturity locus area within studies is actually consistent with the observed models of phenotypic correlations. 01) with quite a few faculties overlapping the same genetic standing (Fig. 1). For instance, EBID11 and you can VWID11 scores both displayed a powerful bad correlation which have MAT95ID and MAT120ID. Which trend get give an explanation for biggest perception QTL having after maturity directly from the postponed onset of VW and you can EB, or simply, early readiness causing very early start of VW and you will EB. A fairly higher relationship was also noticed ranging from vine readiness and gains routine, that have early readiness of the far more spreading increases routine. In contrast, a reduced correlation (roentgen ? 0.2) ranging from vine maturity and you will each other tuber sugar quantity and you can bud-avoid fry color are in line with different types into QTL outcomes.
Tuber sugar, bud-avoid and you may stem-end fry color
Including chromosome 5, extreme QTLs to own sugar, bud-avoid and stalk-avoid fry color was basically sensed from inside the overlapping regions of chromosomes 4, six, 10, and you can 11. 0 cM with the LOD ratings ranging from cuatro.five to six.0 (Desk 3, Fig. 4). Such QTLs explained between 7.dos and eleven.3% of the feature variance. Studies of various easy models expressed one greatest-suitable design for everybody three faculties, with a double-simplex allele (AAAB ? AAAB) into homologous chromosomes H2 and you will H5 regarding RGR and you may Publicity, correspondingly (Desk 3, More file 8: Profile S4). To own sugar, the fresh B allele try on the highest sugar concentration, if you find yourself getting bud-stop and you can stalk-avoid fry colour, the clear presence of the brand new B allele expressed all the way down reflectance values (Fig. 3b).
LOD score shipment away from QTLs for a few attributes along the twelve potato chromosomes (Chr). Abbreviations are such as Fig. step one
A candidate SNP for a double-simplex configuration on H2 and H5 is solcap_c2_12976 at position 67.4 cM. On the reference PGSC v4.03 Pseudomolecules, this SNP maps to the long arm of chromosome 4 at position 69.1 Mb on the F-box and WD40 domain protein gene (PGSC0003DMG400003339), which is close (< 2 Mb) to several genes associated with starch/sugar inter-conversion as annotated in the potato reference genome, and/or associated with tuber quality traits . They include alpha amylase (AMY-4/1 PGSC0003DMG400007974, AMY-4/2 PGSC0003DMG400009891), hexose transporter (HT-4/3, PGSC0003DMG400009994), invertase (NV-4, PGSC0003DMG400009936), and starch branching enzyme I (SBE I, PGSC0003DMG400009981).
On chromosome 6, significant QTLs mapped at 53 cM for glucose and at 43 cM for FBE and SBE, respectively. The LOD profiles ranged from 5.4 to 7.6 and explained between 10.8 and 16.1% of the trait variance, respectively (Table 3, Additional file 9: Figure S5). The best model for all three traits was a double-simplex configuration with allele effects on H2 and H5. As in chromosome 4, the presence of the B alleles was associated with an increase in glucose concentration and a decrease in reflectance readings (Fig. 3c). There are four candidate SNPs escort services in Birmingham with a double simplex configuration nearby (solcap_snp_c2_33777, solcap_snp_c1_10130, solcap_snp_c2_5769, solcap_snp_c2_56145, c1_3003). All were significantly (P-value < 0.0001) associated with the mean trait values, however, solcap_snp_c2_56145 had on average the highest percent of variance explained (9% to 16%). The SNP solcap_snp_c2_56145 maps at position 51.4 Mb of the potato reference genome on the ATP binding protein gene PGSC0003DMG400033089. This SNP is located in a region (
eight Mb) containing several genes regarding the starch/sugar inter-conversion together with hexokinase (HXK-6, PGSC0003DMG400016521), fructokinase (FRK-6/step one, PGSC0003DMG400027017, FRK-6/dos, PGSC0003DMG400026916), and invertase (INV-na-6/step 1, PGSC0003DMG400026107, INV-6/dos, PGSC0003DMG400033142) genetics.